TRYPTOPHAN DESMOLASE OF NEUROSPORA

The Effects of Gene Change on Tryptophan Desmolase Formation.

The role of tryptophan in the physiology of Neurospora.

The genetics and biochemistry of tryptophan metabolism in the bread mold, Neurospora cras&a, have received considerable experimental attention during the past 20 years. By now, an impressive body of information has accumulated to the extent that the intimate details of the biosynthesise and degradation'-' of tryptophan in Neurospora are well known and also to the extent that the genetics of thi...

A Neurospora mutant deficient in the enzymatic synthesis of tryptophan.

Until recently there has been no demonstrated case in Neurospora in which the loss of activity of an essential enzyme could be attributed to gene mutation. It has been assumed from indirect evidence1 that gene mutations do cause such losses and thereby result in “blocks” of essential biochemical reactions. The products of the “blocked” reactions thus become essential growth substances for the m...

The subunit structure of tryptophan synthase from Neurospora crassa.

Tryptophan synthase of Neurospora crassa was purified to electrophoretic homogeneity from the wild type strain 74A which had been derepressed by the presence of 0.5 mM indoleacrylic acid in the growth medium. The isolated material migrated as a single symmetrical peak in the ultracentrifuge with a sedimentation constant of 6.0 S. Gel filtration on Sephadex G-200 AND CONVENTIONAL SEDIMENTATION E...

An enzyme aggregate in the tryptophan pathway of Neurospora crassa.

reactions specifically involved in tryptophan biosynthesis are under the T?ntrol of four genes in Neurospora crassa (AHMAD and CATCHESIDE 1960). The complexity of the gene-enzyme relationships in this pathway was discovered by AHMAD and CATCHESIDE (1960) and AHMAD, KHALIL, KHAN and MOZMADAR (1964) who demonstrated that lesions at one of these genes, tryp-2, could prevent either the accumulation...